About
Summary
PhD in molecular biology, currently researching human-specific brain evolution with complex 3D models
Positions
Postdoc Oct 2023 -
Center for Molecular Biology of Heidelberg University (ZMBH)
In the past three years of my postdoc, I established a brain organoid lab from scratch. I organized documentation for the GMO S1 and S2 lab work. I optimized guided and unguided brain organoid protocols for human and non-human primate cell lines. To overcome the heterogeneity of organoids, I established mid-throughput quality control pipelines. I charactreized the transcriptional profile of generated organoids with high-throughput snRNAseq and ATACseq. I also performed functional validations via CRISPR knockout experiments in human iPSCs.
Education
Max-Delbrück Center for Molecular Medicine 2016 - 2023
Field of study: Molecular Biology
Degree: PhD
I worked with human-specific endogenous retrovirus, studying its role in the genome protection of human embryonic stem cells. I performed a wide range of RNA interference and CRISPR experiments, optimised PCR assays for challenging repetitive sequences and perfromed luciferase assays.
Skills
Laboratory setup: S2 organoid lab infrastructure, SOPs/manuals, QC workflows, cell line acquisition and banking, GMO project documentation for regulatory approval. Organoids and cell culture: Human and primate iPSC-derived cortical and cerebellar organoids, organoid transfections, cryosectioning, wide-field/confocal microscopy, whole-organoid imaging. Gene editing: Plasmid cloning, CRISPR/Cas9 and Cas13 editing, shRNA knockdown, stem cell transfection/electroporation, AAV design and production. Molecular assays: Advanced PCR for challenging templates, nested/gradient/touchdown PCR, gene expression and protein assays, RNA-protein immunoprecipitation, luciferase assays. NGS: 10X snRNA-seq and scATAC-seq sample preparation, multiplexed snRNA-seq, bulk and circRNA-enriched library preparation. Data analysis: R, command line, ImageJ/Fiji. Teaching: Hands-on practicum training and supervision of thesis students.
Keywords: AAV gene therapy, CRISPR-Cas9 genetic editing, iPSC differentiation, laboratory management, microscopy, organoids, PCR, QC , RNAi, RNA-SeqCV
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