Amnah Ahmed

Summary

I am writing to express my keen interest and passion to deliver in the advertised position in Molecular biology.

International Research Working Experience Slovakia-
In Chemickey Ustav SAS, Bratislava, I worked as an assistant on project and was working on the pharmacological activity of some natural Nitric oxide-releasing compounds and checked their cytotoxicity in vitro on HUVEC and VH10 fibroblast cells and in vivo mouse model.

We evaluated cytotoxicity and applicability of novel fluorescent reporters designed for simultaneous multicolour detection of reactive nitrogen (RNS) and reactive oxygen species (ROS) at the cellular level. The cytotoxicity was evaluated by standard assays (such as MTT, neutral red uptake assay or cell cycle analysis) in the cellular models (microglia, fibroblasts, endothelial cells). The probes specificity was determined in cell and cell-free systems exposed to different ROS and RNS as well as in the systems simultaneously generating these species using fluorescence-based methods. The probe applicability was validated in such cellular models as microglia or brain microvascular endothelial cells. The methods based on novel molecular logics was compared with standard methods of detection of RNS and ROS and confronted with biological consequences and signalling pathways of RNS and ROS generation (such as the expression of iNOS protein or proteins involved in the regulation of the endothelial function).

Skills learnt from the last position-

I learnt time management, needed to prioritize my own workload. Designing and implementation of new experimental protocols

Ability to deliver the project within deadline along with my team member learnt team management skill Experimental techniques used to check cytotoxicity of compounds in-vitro and in invivo model Germany-

In 2018, I was working as an internee at Otto von Guericke University, Magdeburg Germany. As an internee, I worked on different molecular biology techniques in a group. I worked on the project of atherosclerosis and we were evaluating hyperglycemia-induced maladaptive UPR-signaling causes cellular senescence and EndoC barrier disruption. The associated SASP and ROS generation promote plaque instability. I successfully established aortic arch atherosclerotic cloning in mice.

 Skills gained in Germany-

I learnt the technical skills mentioned in the mandatory skills section, which enhance my ability to effectively produce my

Input to research and scientifically computation.

 Through my working experience, I learnt to deliver the presentation in front of large groups.

Pakistan- 

I have more than 2 years of research experiences in different national labs of well-known groups and worked on different projects in different capacities as also being a researcher in Quaid-i-Azam University, Islamabad on Molecular Biology in the field of molecular stress and pharmacology with Biological science for 24 months under the supervision of Prof. Dr. Muhammad Rashid Khan. Moreover, I worked on pharmacological activities of plants on rat model during M.Phil studies and then 6 months work experience as a lecturer in HIT degree college Taxila Cantt.

During my M.Phil studies, I completed a thesis degree project (one year project in the project I worked on “Evaluation of Sapindus mukorossi Gaertn For In vitro Cytotoxicity And Hepatoprotection Against CCl4 Induced Toxicity In Rats”. The aim of the project was to assess the antidiabetic, antihemolytic, aphrodisiac, CCL4 induced toxicity in rats. We hope to establish if differences exist in their respective abilities to exhibit some pharmacological actions since they come from various locations of the country.

Conferences attended throughout my career-

§  Seminar presentation on genomic technologies in Leipzig

§  Poster competition in Magdeburg

§  Poster presentation in Jena

§  Poster presentation in Dublin

§  Application of Statistics in Biology (3 days workshop) in Pakistan

§  Participation 2 days conference on “Use of non-edible wild Seeds as Biofuels in Pakistan

§  Medicinal plants used to treat Hypertension among the local Communities of Pakistan

Mandatory Skills:
I have expertise in General laboratory protocols for working in Molecular biology laboratory, Cell culture, DNA and RNA isolation and purification, Fluorescent microscopy, Immunohistochemistry, ELISA, chromatography technique, DNA extraction, Plasmid DNA preparation, and qRT-PCR, Agarose gel electrophoresis, Senescence associated B- Gal staining, BODIPY staining, DAPI and DSF staining, western blotting, HPLC and little knowledge of Next-generation sequencing. The techniques Spectrophotometry, flow cytometry, protein purification, using a fluorescent microscope for DNA damage analysis. I have also experienced working with animal model-like rats, mice, pigeons, Rabbits and earthworms as well.

Computational Skills-
Working with Operating system: MS Windows 2010, Microsoft Word, Excel, Powerpoint Scientific Computational tools included: SPSS, Python, Graph Pad Prism, and Picasa

I would love to work as a Molecular Biologist. As my whole experience revolves around molecular biology, pharmacology, biochemistry, histology, therapeutics and working with mice model and cell culture models in a sterile environment. I am sure; I will be able to use my skills to the full extent during my work period and believe that I could make a contribution in clinical trials. I am highly motivated and committed to working with a great team.

Positions

Education

Skills

Professional interests

I am a passionate, committed researcher with a solid background in molecular biology, pharmacology, biochemistry, histology and therapeutics. I am interested in developing a career that combines research, technology and industry. I am sure; I will be able to use my skills to the full extent during my work period and believe that I could make a contribution to the company.

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Contact details

New opportunities

Open to new opportunities: Yes