About
Summary
I have a PhD in Structural Biology and a Master's Degree in Biophysics. I've worked with protein models of Galectins, glycoproteins related to cancer and metastasis processes in the cell, and have a strong background in X-ray crystallography, SAXS, protein expression and purification, primer design and construction, as well as work with small animals in disease models.
Positions
Postdoc Jun 2019 -
Protein expression and purification. Design and optimization of crystallization conditions. Resolution of structures through crystallographic methods.
Clinical Consultant Nov 2017 - Dec 2018
In Becton Dickinson Mexico Hub, I provided consultancy services for health-care professionals in the use and application of biomedical equipment.
Master's student Sep 2014 - Jul 2015
Faculty of Physical Sciences, Universidad Complutense de Madrid
Biophysical characterization of cellular processes.
PhD student Sep 2013 - Oct 2017
Center for Biological Research (CIB), Spanish National Research Council (CSIC)
Structural and biophysical determination of apoptosis- and metastasis-related proteins Galectins. Gene and primer design, protein expression and purification and several crystallization methods for Galectin-Related Protein and Galectins.
Pregrad researcher Dec 2011 - Apr 2013
Autonomous University of Puebla
Small animal models (rats) for inflammation and disease research. Fully complying with humane guidelines, I have performed surgery, euthanasia and organ extraction for protein studies in alkaline phosphatase and heavy metal influence on hepatic markers like bilirrubins, alkaline phosphatase and metallothioneins.
Education
Complutense University of Madrid 2014 - 2015
Field of study: Biophysics
Degree: Master's Degree
Autonomous University of Madrid 2013 - 2017
Field of study: Molecular Biosciences
Degree: Doctoral Degree
Structural characterization of Galectin-3 and Galectin-Related Protein by X-ray crystallography
Autonomous University of Puebla 2006 - 2012
Field of study: Medicinal Chemistry
Degree: Honors Bachelor's Degree
Skills
Macromolecules crystallization and optimization methods. Structural resolution of macromolecules by synchrotron X-ray diffraction analysis (X-ray crystallography), small-angle X-ray scattering, circular dichroism and analytical ultracentrifugation.
Data processing and resolution of X-ray diffraction patterns through specialized software (XDS, CCP4i, Phenix, Coot and PyMol) and molecular modelling.
Recombinant DNA design, mutagenesis, genomic DNA isolation and digestion, cell transformation and bacterial/insect cell cultures. Protein purification and characterization techniques, affinity and molecular exclusion chromatography, electrophoresis, FPLC/HPLC.
Animal models (handling, inoculation and basic principles of surgery) for experimentation, complying fully with most current humane treatment guidelines. Preparation of mammalian cells cultures.
Statistical analysis with Excel and R.
Medicinal chemistry techniques in the area of biochemistry and hematology.
Professional interests
I am deeply interested in the elucidation of protein structures that can serve as basis for the development of novel pharmacological approaches, as well as environmentally friendly energies. I am experienced in structural biology techniques like X-ray crystallography, SAXS CD and AUC, molecular modelling, and in molecular biology techniques like gene design and protein expression in microbiological vectors. Most of my work is linked with metastasis-related proteins, Galectins, and am currently working with Deubiquitinases activated in processes of Glioma/Glioblastoma malignancies.
CV
Please sign in to view the CV.