BAKER AL-SHARA

PhD student Sep 2014 - Jun 2019

Institute of Biological Sciences, University of Malaya

lecturer Sep 2011 - Aug 2014

Biological Sciences, University of Hail

Responsibility
Teaching:
A. Plant taxonomy lab, helping student to understanding the basics of taxonomy and
learn vegetative and reproductive features and terminology that are useful in the
identification of flowering plants. Helping student to recognize some of the common
and unusual families of flowering plants in Saudi Arabia. In addition, helping student
to drawing floral diagram and longitudinal section (LS) from different families of
flowering plants.
B. Plant anatomy lab, helping student to understand the arrangement of tissue and cells
types within the dermal, ground, and vascular tissue systems in vascular plants. Helping
student to recognized specialized cells and their components. helping student to
identifying the five differences between monocots and eudicots and be able to
recognize these key features (macroscopically and microscopically) in the plants and
prepared slides you observe in lab. Also, helping student to identify a cross section and
the specific tissues of herbaceous eudicot, herbaceous monocot, and woody eudicot and
be able to state the functions of those tissues
C. Plant microtechnique, help student in preparing fixative to preserved plant samples.
Help student in preparing slides prepared from different parts of plant (dehydration and
clearing, mounting, Embedding and sectioning). Help student in optimize the different
staining protocols.
D. Plant Physiology, helping student to understand the main physiological processes in
plant such as; photosynthesis, ceullar respiration, diffusion, osmosis, ...etc

Researcher Oct 2008 - Aug 2011

Biological Sciences, Yarmouk university

A. Preparing different stock solutions (macronutrient, micronutrient, plant growth
regulators, vitamins, iron and other organic supplements such as amino acid)
B. Preparing different types of media
C. Sterilization different parts of plant such as seeds and leaves
D. Culturing different parts of plant in vitro
E. Monitoring and reading results
F. Help in experiments, writing and analysis results in manuscript that published in Plant
Cell, Tissue and Organ Culture
Al Khateeb, W., Hussein, E., Qouta, L., Alu’datt, M., Al-Shara, B., & Abu-Zaiton, A.
(2012). In vitro propagation and characterization of phenolic content along with
antioxidant and antimicrobial activities of Cichorium pumilum Jacq. Plant Cell, Tissue
and Organ Culture (PCTOC), 110(1), 103-110.

Preparation samples for light microscopy
A. Fixation, samples fixation from different tissues (plant and animal tissues) by
using different fixatives glutaraldehyde and formalin alcohol acetic acid (FAA)
for plant tissues. Bouin’s, osmium tetroxide and phosphate buffers for animals’
tissues
B. Dehydration process, using different gradient of acetone and tertiary butyl alcohol
for dehydration plant tissues and ethanol for dehydration animal tissues
C. Infiltration and embedding by using Xylene and Toluene for infiltration and wax
(Easter wax, paraffine) for embedding
D. Sectioning process, by using microtome
E. Staining process, using different type of stain such as toluidine blue stain, safranin
and fast green for plant tissues and eosin and hematoxylin for animals’ tissues
Preparation samples for scanning and transmission electron microscopy
A. Fixation, samples fixation from different tissues (plant and animal tissues) by
using different fixatives glutaraldehyde for plant tissues. osmium tetroxide and
phosphate buffers for animals’ tissues
B. Dehydration process, using different gradient of acetone for dehydration plant
tissues and animal tissues
C. Infiltration and embedding by using Xylene and Toluene for infiltration and resin
for embedding
D. Sectioning process, by using ultramicrotome (diamond knife)
E. Staining process, using lead citrate stain
F. Help in experiments, writing and analysis results in manuscript that published in
Journal of Toxicology Review
Khalil, A., Al-Adhammi, M., Al-Shara, B., Gagaa, M., Rawshdeh, A., & Alshamli,
A. (2012). Histological and ultrastructural analyses of male mice exposed to mobile
phone radiation. J. of Toxicology Review, 1(1), 1-6.

University of Malaya 2014 - 2019

Field of study: Plant Biotechnology
Degree: Ph.D.

Project 1: In vitro propagation of Carica papaya L. cv. Eksotika via somatic embryogenesis
In this project I studied the root formation problems during in vitro propagation of Carica papaya
using somatic embryogenesis from immature zygotic embryos. Firstly, I was standardized the
protocol for fruits and seeds sterilization. Then I was standardized the induction, maturation,
germination (shoot formation) media by examined different media type, auxin (2,4-D)
concentrations, other additives such as polyethylene glycol, phloroglucinol, activated charcoal.
For root formation phloroglucinol and riboflavin I was used with vermiculate to supporting media.
Finally, I was transferred the explants for acclimatization. Also, the synthetic seed formation was
standardized by examined different concentrations of sodium alginate with different solvents,
calcium chloride concentration and different time of exposing sodium alginate matrix to calcium
chloride. After that I was studied the developmental stages of somatic embryogenesis by using
light microscope to describe and tracking the root formation in early stage. In conjunction with
histological study I was studied the genes expression level for LAX2, LAX3 and PIN4 genes during
development of somatic embryos. These genes play acritical role in transfer auxin (2,4-D) during
root development of somatic embryos. This project presents some publication some of these papers
was published other still under process:
1- Al-Shara, B., Taha, R. M., & Rashid, K. (2018). Biotechnological methods and limitations of
micropropagation in papaya (Carica papaya L.) production: a review. Journal of Animal and
Plant Sciences, 28(5), 1208-1226.
2- Baker Al-Shara, Rosna Mat Taha, Jamaludin Mohamad Hashimah Elias and Asif Khan (2018)
Induction of somatic embryogenesis from Carica papaya L. ‘Eksotika’.  In Process.
3- Baker Al-Shara, Rosna Mat Taha, Jamaludin Mohamad Hashimah Elias and Asif Khan (2018).
In vitro regeneration and artificial seed production from somatic embryos of Carica papaya
‘Eksotika’. In Process.
4- Baker Al-Shara, Rosna Mat Taha, Jamaludin Mohamad Hashimah Elias and Asif Khan (2018).
Somatic embryogenesis in Carica papaya ‘Eksotika’- Histological and Molecular study. Under
writing manuscript.

Yarmouk university 2006 - 2009

Field of study: Biology
Degree: Master Degrees

Yarmouk university 2001 - 2006

Field of study: Biology
Degree: bachelor degrees