About
Summary
- Molecular and Infection biologist with a project management background
- Extensive international work experience in multicultural environments
- Keen interest and experience in training colleagues and juniors
Positions
Post-doctoral researcher Jan 2015 -
Advisor: Prof. Lars Dölken
- Established a novel pull-down strategy for miRNA-mRNA interactions
- Generated transgenic mice with organ specific expression of a recombinant Argonaute protein
- Prepared small RNA libraries using Illumina SMART and TruSeq technologies
- Analysed small RNA sequencing data to design AntagomiRs
- Actively involved in organizing lectures and practical courses for bachelor and master students
- Lab management
Post-doctoral researcher Jan 2015 - Jul 2015
Department of Medicine, MRC Laboratory of Molecular Biology, University of Cambridge
Advisor: Prof. Lars Dölken
- miRNA-RNA interaction studies in cytomegalovirus infected murine cells
- Later moved with my lab to the University of Würzburg (Germany), where I continued with my project
Post-doctoral researcher Sep 2013 - Dec 2014
Advisor: Prof. Reza Salavati
- Found an involvement of an AU-rich element (ARE) binding protein DRBD13 in the differentiation process of sleeping-sickness causing parasite Trypanosoma brucei
- Trained master and PhD students
- Lab management
Research Assistant Sep 2007 - Aug 2008
Supervisor: Prof. Pawan Malhotra
- Bioinformatic prediction of the presence of small RNA molecules in malaria parasite Plasmodium falciparum
- Verified the presence of the small RNA molecules by Northern blotting
- Learned Biosafety Level 2 work (BSL-2)
- Helped PhD students and Post-doctoral researchers in general lab work, eg. Western blotting, cell-cluture, plasmid mini and midi-preparations.
Education
University of Heidelberg, Germany 2008 - 2013
Field of study: Molecular Biology
Degree: Doctorate
Supervisor: Prof. Christine Clayton
- Studied functional roles of two RNA-binding (PUF2 and PUF5) of sleeping-sickness causing parasite "Trypanosoma brucei"
- Performed pull-down experiments to find the target RNA molecules of the two proteins
- regularly organized practical courses for bachelor and master students (molecular and cell biology & infection biology)
Jawaharlal Nehru University, New Delhi, India 2005 - 2007
Field of study: Life Sciences
Degree: Master of Science
Skills
Cell culture (adherent and suspension; mammalian cells and parasites: bio-safety level II), transient transfection (electroporation, lipid and calcium phosphate mediated), stable cell line generation using plasmids and lentiviral vectors, virus purification and titration, virus mutagenesis, cloning and expression of genes, nucleic acid purification, polymerase chain reaction (including qPCR), gene transfections, reporter gene assays, blotting techniques (Western, Northern and Southern), RNA and microRNA handling, RNAi, miRNA transfection, small RNA library preparation, Protein purification and quantitation, Immunoprecipitation (IP), Co-IP and PAR-CLIP, ELISA, Flow-cytometry, fluorescence microscopy including confocal microscopy and FISH, RNA-seq data analysis, in silico cloning and sequence analysis software programs: SnapGene, Geneious, DNASTAR Lasergene, MS office suite, MS Project, Adobe Photoshop, Illustrator and Lightroom, GraphPad Prism, Endnote, Project Management, English (fluent), German (fluent), Hindi (mother tongue), Spanish (basic)
CV
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