About
Summary
I am an accomplished analytical chemist with over a decade of experience, including five years of postdoctoral research, specializing in mass spectrometry-based metabolomics, natural product drug discovery, and biosynthesis. My expertise spans LC-MS, GC-MS, NMR, and spectroscopy techniques, with proficiency in instrument operation, troubleshooting, data acquisition, and analysis. I have extensive hands-on experience with UPLC-Q-Exactive-Orbitrap-HRMS (Thermo); GC-Q-TOF-MS, UPLC-Q-TOF-MS, HPLC-TOF-MS (Agilent); HPLC (Agilent, Waters, Buchi); MPLC, and Combi-Flash (Buchi). My research has advanced plant-pest interactions through mass spectrometry applications in detoxification enzyme studies and RNA interference (RNAi). I also specialize in extraction, purification, and structure elucidation of bioactive compounds.
I am proficient in Agilent’s MassHunter, OpenLab; Thermo Scientific’s Xcalibur, TraceFinder, Freestyle, Compound Discoverer; Waters’ Empower, MassLynx; and metabolomics tools like MZmine, MetaboAnalyst, XCMS, MS-DIAL, and GNPS. Skilled in analytical method development and validation, I have a strong publication record, conference presentations, and successful research grants. Passionate about mentorship and collaboration, I integrate cutting-edge analytical techniques to solve complex challenges in biochemistry, pharmaceuticals, and natural product research.
Positions
Postdoctoral Associate Jan 2022 -
Biology, University of Western Ontario
• Engaged in both targeted and untargeted metabolomics investigations, specifically exploring plant-pest interactions.
• Connecting Mass Spectrometry with RNA interference (RNAi) based studies to advance bio-miticide development.
• Proficiently managed, troubleshoot and maintained advanced instruments, including LC-TOF-MS, GC-TOF-MS, and UPLC-Q-Exactive-Orbitrap-HRMS.
• Specialized in analyzing tryptophan-derived metabolites, employing MS/MS techniques for identifying glucosinolates and their breakdown products, as well as phenylpropanoids.
• Demonstrated expertise in processing MS data through tools such as Metaboanalyst, MS Dial, and MZmine for various analytical purposes.
• Applied metabolic networking techniques using the GNPS platform.
Research Scientist Feb 2021 - May 2021
Analytical Chemistry, Enveda Therapeutics India Pvt. Ltd.
- Developed methods for data acquisition and Data analysis for biological samples
- Purification and Quantitative estimation of compounds through HPLC
- Solid Phase Extraction of plant extracts through different matrixes and SPEmanifold
- Targeted/untargeted, in-vitro, and in-vivo PK/PD metabolic sample analysis.
- QC/QA analysis, data exporting, and reporting for HPLC
Research Associate Jul 2019 - Mar 2020
Organic Chemistry, CSIR-National Chemical Laboratory
- Exploration of qualitative and quantitative metabolomics profiling on volatiles using GC and GC-MS techniques
- Precise quantification of sugar-mimicking alkaloids/imino-sugars in mulberry varieties using advanced UPLC-Q-Exactive-Orbitrap-HRMS technology
National Postdoctoral Fellow Jun 2017 - Jun 2019
Organic Chemistry, CSIR- National Chemical Laboratory
- Conducted comprehensive metabolomic investigations of phytoestrogens using advanced analytical techniques including GC-MS, GC-Q-TOF-MS, HPLC, MPLC, Combi-flash, UPLC, LC-MS, and UPLC-Q-Exactive-Orbitrap-HRMS
- Explored biosynthetic pathways and performed comparative transcriptomics analysis on phytoestrogens
- Evaluated the anti-osteoporosis and antioxidant properties of natural products
Education
Jawaharlal Nehru University (Central Drug Research Institute), New Delhi 2012 - 2016
Field of study: Natural Product Chemistry/Analytical Chemistry/Life Science/Drug Discovery
Degree: Ph.D.
❖ Thesis Title: Bioprospection and in-vitro biosynthesis of bioactive secondary metabolites from Indian traditional plants used for bone healing
● Isolated and purified compounds using various chromatographic methods including normal and reverse phase column chromatography, HPLC, combi-flash columns, and gas chromatography
● Elucidated compound structures through techniques such as NMR, IR, UV spectroscopy, and ESI-MS
● Performed chemical fingerprinting of natural product extracts from medicinal orchids using UPLC-Q-TOF-LCMS and DART-MS
●Detected and quantified phytoestrogens in cell cultures using UPLC-Q-TOF-MS
● Evaluated the anti-cancer, antioxidant, and anti-osteoporosis activities of traditional medicinal plants
● Development of quality control markers for herbal drugs
University of Rajasthan, Jaipur, India 2009 - 2011
Field of study: Botany with specialization in Advance Plant Physiology and Biochemistry
Degree: Master Of Science (M.Sc)
Specialization in Advance Plant Physiology and Biochemistry
University of Kota, Kota, India 2005 - 2008
Field of study: Chemistry, Botany and Zoology
Degree: Bachelor Of Science (B.Sc.)
Skills
Untargeted & Targeted Metabolomics
LC-MS, MS/MS, Tandem Mass Spectrometry
NMR, IR And UV-Vis Spectroscopy
Enzyme Chemistry, Detoxification Enzymes YCfP450,GSTs, UGTs and Methyl Transferases
RNAi
MS data processing tools like MZmine, Metaboanalyst 6.0, 'XCMS, MS Dial, and GNPS
Statistical Analysis through MS Excel, Graphpad-Prism, Metaboanalyst 5.0 and R
R Programming
Project monitoring tools like E-notebooks, Benchling, 15-five, and Slack
Professional interests
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Keywords: Chromatography, Exposomics, FT-IR, GC-MS , LC-MS/MS, Lipidomics, Mass spectrometry, metabolomics, NMR, QA, QCCV
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