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Cristian Nogales

Cristian Nogales

Main current position is not set

  • Joined: 2017
  • Items posted: 0
  • Profile views: 206

About

Summary

I am a recent postgraduate in Biotechnology at the Autonomous University of Madrid with a wet lab and a computational biology background.

Recently, during my Master Thesis, I had the opportunity to work in a molecular biology laboratory studying the expression patterns of cis-regulatory elements (CREs) in Drosophila melanogaster imaginal discs. During that time, I earned hands-on experience in general molecular biology techniques such as molecular cloning, DNA isolation and confocal microscopy.

Moreover, bioinformatics has been one of my favourite topics since I got introduced to it in my third year at college and, during my final project at university, I became familiar with RNA-seq data analysis tools and linux.

I love challenges and learning new things, so if you think I could be helpful to you in any way, feel free to contact me directly at [email protected] (✉).

Positions

Master Thesis Student Jan 2016 - Jun 2016

Severo Ochoa Molecular Biology Centre (CBMSO), Spanish National Research Council (CSIC)

Accomplishments:

  • Performed molecular cloning and bacterial expression of Drosophila melanogaster cis-regulatory elements in GFP reporter vectors.
  • Dissection and staining of fly imaginal discs.
  • Obtained Z-stacks of high-resolution images of fly imaginal discs, employing a confocal microscope and analysed them using ImageJ and Photoshop.

Student Intern Nov 2012 - Sep 2014

University of Sevilla

Accomplishments:

  • Analysed RNA-seq data with R and Bioconductor by following the TopHat and Cufflinks protocol.
  • Assembled a gene co-expression network for Oryza sativa, commonly known as Asian rice.
  • Identified and functionally characterised gene expression patterns and hub genes, as well as potential biotechnological genes, studying their functions and potential regulation.

Education

Autonomous University of Madrid 2015 - 2016

Field of study: Life Sciences
Degree: Master's Degree in Biotechnology

Relevant Courses:

  • Applied Statistics for Molecular Biosciences
  • Genetic and Protein Engineering
  • Biotechnology of Microorganisms
  • Bioprocess Engineering

University of Seville 2010 - 2014

Field of study: Life Sciences
Degree: B.Sc. in Molecular and Applied Biochemistry

Relevant Courses:

  • Molecular Systems Biology
  • Molecular Genetics and Genetic Engineering
  • Structure of Macromolecules
  • Synthesis of Macromolecules
  • Neurochemistry: Neurodegenerative Processes
  • Virology

Skills

molecular cloning, PCR, DNA extraction and quantification, electrophoresis, site directed mutagenesis, microscopy, confocal microscopy (LSM 510), Drosophila melanogaster, RNA-seq data analysis, python, R, bioconductor, RStudio, shell scripting, linux, MS Office, ImageJ, verbal and written communication, organizational and interpersonal skills

CV

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Contact details

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New opportunities

Open to new opportunities: Yes

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