About
Summary
Protein biochemist / biophysicist. I have been working several years in the biophysical, structural and functional characterization of proteins. During my PhD I studied, among other similar proteins, Skl, a Streptococcus mitis phage endolysin with potential enzybiotic activity against Streptococcus pneumoniae. Also we designed, constructed and characterized chimeric proteins based on Skl, with the aim to better understand the role of each protein module in the antibacterial activity and to create new promising enzybiotics.
Education
Universidad Autónoma de Madrid
Field of study: Biophysics
Degree: PhD
PhD in Biophysics — Autonomous University of Madrid (UAM), Spain and Institute of Physical Chemistry “Rocasolano”, Spanish Council of Scientific Research (IQFR-CSIC)
Thesis: “New antimicrobials based in lytic enzymes from Streptococcus mitis and Streptococcus pneumoniae.” (Nuevos antimicrobianos basados en enzimas líticas de Streptococcus mitis y Streptopcoccus pneumoniae ).
Skills
Molecular biology: cloning, transformation and expression in E. coli; recombinant protein expression and purification (chromatography, SDS-PAGE, UV-visible spectroscopy, etc.);
Biophysical protein characterization: Differential Scanning Calorimetry (DSC), Circular Dichroism (DC), Analytical Ultracentrifugation, Chromatography: HPLC and FPLC.
Structural protein characterization: Small Angle X-Ray Scattering (SAXS) data colecting and processing to obtain low resolution protein models, programs and resources to obtain and evaluate high resolution protein models: databases, structure prediction programs, sequence alignment, modelling and visualization.
Bacterial cultures and lysis essays.
Professional interests
Protein characterization, protein engineering, enzybiotics.
Protein design and applications.
Synthetic biology.
CV
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