About
Summary
I would be happy to brief my background here; I did my Master’s in Biotechnology from Allahabad Agricultural Institute-Deemed University, Allahabad, India in 2006. Several of my academic courses were Plant Biotechnology, Molecular Biology, Genetic Engineering, Cell biology and Animal Biotechnology etc. During Master’s thesis, I worked on research area “Plant Molecular Biology” and I studied the “Induction of Hairy Roots from Catharanthus roseus and its Molecular & Phytochemical Characterization”, under the supervision of Late Dr. Jayanti Sen, Staff Scientist, National Institute of Plant Genome Research, New Delhi, India.
After completion of my Master’s, I worked as Junior Research Fellow under the supervision of Dr. Alok Krishna Sinha, Associate Professor, National Institute of Plant Genome Research (NIPGR), New Delhi, India, from September, 2007-June 2010. Here, I was involved in several projects like as “Molecular and Chemical characterization of different populations of Podophyllum hexandrum and preparation of tissue specific as well as Subtractive EST databases towards the understanding of Podophyllotoxin biosynthesis”, “Functional characterization of rice mitogen activated protein kinase 7(OsMPK7) to study its role in abiotic stress tolrence” and “Rice WNK1 is regulated by abiotic stress and involved in internal circadian rhythm”. My contribution in above mentioned project fetched me as an authorship in the article. “Rice WNK1 is regulated by abiotic stress and involved in internal circadian rhythm (Kumar et al, 2011) and Expressed sequence tags and molecular cloning and characterization of gene encoding pinoresinol/lariciresinol reductase from Podophyllum hexandrum (Wankhede et al, 2013). I also submitted full length nucleotide sequence of Sinopodophyllum hexandrum pinoresinol/lariciresinol reductase in NCBI GenBank Database.
Next, I joined the Plant Molecular Biology group under the supervision of Dr. Narendra Tuteja, Group Leader at the International Centre for Genetic Engineering & Biotechnology (ICGEB), New Delhi, India from July, 2010 – February 2012. I worked as Senior Research Fellow in the project entitled “Development of Herbicide & Stress tolerant Transgenic Onion”. Herbicide resistance refers to the inherited ability of a weed or crop biotype to survive an herbicide application to which the original population was susceptible. Due to this aspect I developed genetically modified transgenic herbicide tolerant onion as well as rice plants from the rice embryogenesis method.
I got Milgat Hanasi (President Fellowship), Israel to do research at Bar-Ilan University under the supervision of Prof. Shulamit Michaeli from April, 2012 – Feburary 2013. I was involved in the project entitled “Spliced leader RNA silencing (SLS) – a programmed cell death pathway in Trypanosoma brucei that is induced upon ER stress”. Here, I studied the Splice leader RNA silencing (SLS) is a programmed cell death (PCD) pathway that is utilized by the parasites to substitute for the apoptosis observed in higher eukaryotes under prolonged ER stress. In this study, we demonstrate that despite the lack of transcriptional regulation, procyclic trypanosomes change their transcriptome as a response to ER stress by differential mRNA stabilization. This work will publish soon. Next, I was worked on another project and got an authorship in article “The hnRNP F/H homologue of Trypanosoma brucei is differentially expressed in the two life cycle stages of the parasite and regulates splicing and mRNA stability (Gupta et al, 2013).”
My combined skills in Plant Molecular Biology, RNA Biology, Genetics, Biochemistry, and Microbiology to put me in a strong position to perform efficiently in this particular area of research. I am flexible, sincere and have been working on more than one project by my prioritization of works and amenability to my colleagues which has made me favorable for their collaborative research, I think It would be an added advantage on my PhD candidature here. I will eagerly be waiting for your kind response and wish to hear from you soon. I have enclosed my CV for your review. I am thankful for your kind consideration.
Your’s sincerely,
Dipul Kumar Biswas
MSc Biotechnology
Positions
Senior Research Fellow Feb 2007 - Jul 2015
National Research Centre on Plant Biotechnology, IARI, Pusa Campus, New Delhi., National Institute for Plant Genome Research
Research Experience
2015-05-04 - 2015-07-31
2014-02-01 – 2015-03-22
2012-04 - 2013-01
2010-07 - 2012-02
2007-09 - 2010-06
2007-02 - 2007-07
National Research Centre on Plant Biotechnology, IARI, Pusa Campus, New Delhi.
Position: Senior Research Fellow
Supervisor: Dr. Basavaprabhu L. Patil, Senior Scientist (Agricultural Biotechnology), NRCPB.
Project: Cloning and Charachterization of Pigeonpea sterility mosaic virus (PPSMV) .
Department of Plant Molecular Biology, Delhi University, South Campus, New Delhi.
Position: Senior Research Fellow
Supervisor: Dr. Surekha K. Agarwal, Assistant Professor, DPMB, Delhi University.
Project: Expression profiling and functional characterization of wheat miRNAs.
Faculty of Life Sciences, Bar Ilan University, RamatGan, Israel. Position: Research Fellow
Supervisor: Prof. Shulamit Michaeli, Professor, RNA Biology and Parasitology.
Project: I was involved in 2 different projects as follows: “Spliced leader RNA silencing (SLS) – a programmed cell death pathway in Trypanosoma brucei that is induced upon ER stress” and T. brucei hnRNP F/H homologue regulates both mRNA stability and splicing, at Bar Ilan University, Israel.
Plant Molecular Biology Group, International Centre for Genetic Engineering and Biotechnology (ICGEB), New Delhi, India Position: Senior Research Fellow
Supervisor: Dr. Narendra Tuteja, Senior Scientist, Plant Molecular biology Group, International Centre for Genetic Engineering & Biotechnology (ICGEB), New Delhi, India.
Project: entitled “Development of Herbicide & Stress Tolerant Transgenic Onion.”
Supervisor: Dr. Alok Krishna Sinha, Staff Scientist V, National Institute of Plant Genome Research (NIPGR), New Delhi, India.
Project: I was involved in 3 different projects as follows:
1- “Molecular and Chemical characterization of different populations of Podophyllum hexandrum and preparation of tissue specific as well as Subtractive EST databases towards the understanding of Podophyllotoxin biosynthesis.”
2- “Functional characterization of rice mitogen activated protein kinase 7 (OsMPK7) to study its rol
in abiotic stress tolrence.”
3- “Cloning and Characterization of WNK1 from Oryza sativa".
Auroprobe Laboratories, New Delhi. Position: Trainee Scientist
Supervisor: Dr. Nimrat Bawa, Diector of Technical Affairs, Diplomate of American Board of Pathology.
Project: “Diagnosis of MTB, Human papilloma virus, Hepatitis B viruses, Hepatitis C viruses, HSV & HLA from Human Patient Samples”.
Responsibilities: I have worked as research trainee under the Biotech Industrial Training Programme (BITP) sponsored by Biotech Consortium Limited and Department of Biotechnology (Govt. of India). My work focused on diagnosis of human diseases such as: Decontamination of human samples by Modified Patroff Method to isolate Mycobacterium tuberculosis, Isolation of DNA and RNA from human patient samples like as urine, sputum, blood, Menstrual Blood, Semen, and Placenta samples, Diagnosis was done by Nested PCR/ Hot Start PCR/Cobas Amplicor or DNA Hybridization, Culturing of MTB and testing of drug susceptibility through BACTEC460 TB system, Confirmation test using Fast Plaque Assay & ZN staining.