About
Summary
I am a molecular biologist with extensive training in protein biochemistry, structural biology, and cell biology, specializing in DNA replication, mitosis, and DNA repair mechanisms. With a PhD from the University of Copenhagen and postdoctoral experience at the University of Oxford, I have a proven track record in using advanced biophysical techniques (X-ray crystallography, cryo-EM) and genome editing tools (CRISPR, siRNA) to investigate protein interactions that maintain genomic stability. My current research at the University of Southern Denmark focuses on replication stress, cell cycle regulation, and chromatin dynamics, combining proteomics, imaging, and functional genomics to explore their role in cancer and ageing. I aim to drive innovative, interdisciplinary research that addresses key questions in genome integrity and human disease.
Positions
Postdoctoral Research Associate Aug 2021 -
Postdoctoral Research Associate Jan 2017 - Dec 2020
Guest Researcher Dec 2012 - Feb 2013
PhD student Apr 2012 -
Center for Protein Research, University of Copenhagen
Molecular Characterization of PICH protein interacome in Mitosis
Ultra-fine DNA bridges (UFBs) have been identified as one of the threats for genomic stability. UFBs can be only visualized by staining PICH (Plk1-interacting checkpoint helicase) or BLM (Bloom syndrome protein) using antibodies. Knockdown of PICH induces chromosome non-disjunction resulting in intertwined regions of stretched DNA. We are trying to characterize the PICH protein interactome and its biological functions on UFBs during Mitosis
Research Assistant Nov 2011 - Mar 2012
Center for Protein Research, University of Copenhagen
Molecular Characterization of Werner syndrome protein Interactome
Education
Uppsala University 2009 - 2011
Field of study: Applied Biotechnology
Degree: Masters Degree
Protein engineering
Structural Biology
Protein biotechnology
Anna university 2004 - 2008
Field of study: Biotechnology
Degree: Bachelors of Technology
genetics
Immunology
Proteomics and genomics
Animal biotechnology
Chemical engineering
Mass transfer operations
Skills
Overall Laboratory Skills: Molecular cloning, protein purification using mammalian, insect cells and E. coli expression system, radio activity assays, ATPase activity assay, Protein: DNA/Protein: RNA interaction using EMSA, HPLC and FPLC, SDS-PAGE, Western blot, Mass spec sample preparation, Protein characterization using CD (Circular Dichroism) spectroscopy, SPR (Surface Plasmon Resonance), ITC (Isothermal Titration Calorimetry), MST (Microscale Thermophoresis) and MALS (Multi Angle Light Scattering), Crystallization setup, Ubiquitination and Deubiquitination assay, Negative staining (T12), CryoEM sample preparation (Vitrobot FEI MK IV) and CryoEM data collection (Arctica), Cell culture, clonogenic assay, genetic manipulations using siRNA and CRISPR, IP-pulldown for mass spec analysis, High content imaging using QIBC (Quantitative Image Based Cytometry) and Confocal microscopy.
Bioinformatics and Computational Skills: ScanR software package with AI assisted image analysis, Alphafold prediction analysis, Graph pad Prism, ImageJ, Evil Fit, BIAcore Evaluation software, ChimeraX, PyMol, EMAN2, Adobe Illustrator, Endnote X9, Mendeley, MS Office and Excel.