Jose Manuel Liñares Tablante

Summary

Mi goal was always to become in someone valuable for the society and I think that we are valuable in the extent that we put our knowledge and abilities to help people.
Mi name is Jose Manuel Liñares, I am a Cellular Biologist from the Universidad Central de Venezuela,  in 2004 I started mi laboratory training with Prof. Ph.D Gustavo Benaim  where I learned the basis of scientific method and also basic tools as well as some molecular biology and biophisics techniques. In 2005 the Ph.D Graciela Uzcanga together with G. Benaim offered me a thesis working with T. evansi, in this work we show the presence of a Ca2+ channel in the plasma membrane of these parasites with characteristics similar to the human L-type voltaje-gated Ca2+ channel never describe in T. evansi, as well we show that a well know antiarrhytmic Amiodarona was capable to affect the acidocalcisomes, and also collapse de electrochemical mitochondrial potential, thus inducing the release of Ca2+ to the cytoplasm. Other interesting finding obtained in the course of this work was that sphingolipids (sphingosine and ceramide) were able to activate T. evansi plasma membrane Ca2+ channels. Sphingosine (Sph) is known to inhibit many human enzymes, such protein kinase C and several Ca2+-calmodulin dependent enzymes. Concerning Ca2+ movements, it has been demonstrated that Sph inhibits the human plasma membrane Ca2+-ATPase, also Sph increase the intracellular Ca2+ concentration in several cell. However at the time there is no report of an action of Sph in T. evansi parasites. This work was awarded of the maximum grade and it was suggested his publication with a few modifications, but mi political convictions made this impossible. This made me take as a first graduate job in 2007 until 2011 a position as teacher, this I had to say was an enriching experience which allowed me to deal with group management and coaching, minimize one of my biggest fears of public speaking and express my ideas, among other valuable teachings.

After that period I was accepted in the official Master of Genetics and Cellular Biology in the Alcala University. During that period I took courses like Cellular aging; current perspectives in cells biology; structure, organization and evolution of genomes and Stem Cells biology and it was during this subject when I meet Ph.D. Helena Mira Aparicio and Ph.D. Pilar Sanchez Gomez at the Instituto de Salud Carlos III, this was the beginning of my “tesina” under her tutorial, during this period I received intensive training on cell cultures, oncolytic adenovirus handling and safety procedures, transfections, manipulation of NUDE mice for: intragastric and intravenous treatments, xenografts generation, maintenance of neural stem cells and from SVZ, brain perfusion for histological analysis; isolation of RNA, cDNA, PCR and qPCR analysis, immunofluorescence staining and immunohistochemistry analysis,. This led my work title “Effects of the ICOVIR series on the growth of glioblastomas. Using stem cells as cellular vehicles”, this work was awared of the second maximum grade among my peers. In this work we were able to deliver the adenoviruses (created by the group of Ph.D. Ramon Alemany) in the interior of neuronal stem cells who migrate to the tumoral zone and delivered de adenoviruses on mouse brains and even more we were able to demonstrate the potential use of one of the adenovirus tested on the extra cellular matrix of the tumor which could lead to increase the therapeutical response. Other interesting finding obtained in the course of this work was that we were able to produce a model closer than the pattern observed in patients with Glioblastoma. Worth mentioning that these adenoviruses are being used now as part of a bigger project financed by the Spanish Government on the “Plan E”, Subprogram for the Promotion of International Scientific Cooperation, granted to Ph.D Javier Garcia Castro. In the same period of time I was able to participate as a co-author of a paper titled: DYRK1A modulates EGFR Stability and Tumor Growth in Receptor-Dependent Glioblastomas. J Clin Invest. Jun 3;123(6):2475-87.
In 2013 after I finished mi studies I had to return to Venezuela and started as an assistant of Ph.D Hernan Carrasco on the laboratory of Molecular Biology of Parasites in the Institute of Tropical Medicine, Medicine Faculty of the Central university of Venezuela. In his lab I am responsible to determinate the presence of circulating T. cruzi parasites in blood by PCR and parasitological assays form samples collected in several trips around Venezuela, which led to local authorities to start a fumigation and education plan for the communities in Portuguesa and Vargas states to prevent Chagas. This also contributed to the timely treatment of these patients. We are also now leading the first investigation for the presence of triatomine bugs and Chagas disease in the Amazonian of Venezuela
I am also part of a research staff in the IDEA Foundation, under the coordination of Ph.D. Ivan Galindo in the Laboratory of Genomics and Proteomics, currently working on the construction of vectors to evaluate reporter gene expression at the transcriptional level of proapoptotic genes and we are evaluating the ability of induction of apoptosis of various natural compounds in cancer cell lines MCF-7 and PC3.
I am hard-working, intelligent, friendly, open-minded, my perseverance and dedication helped to build my foundation, but my curiosity and affinity for science fuel my passion for learning and face new challenges.

Positions

Education

Skills

PCR, PCR standarizations, real time-PCR, PCR-RFLP, STR analysis by Powerplex-16, microsatellite analysis, perform and analyse sequences, DNA, RNA and cDNA isolation, cell and parasites cultures, MTT analysis, western blot analysis, perform xenografts, Intragastric and intravenous treatments in murine models, brain perfusion in mices for histological analysis, Immunofluorescence staining and immunohistochemistry analysis, IgY antibody purification, biophysics, calcium homeostasis, neuro-oncology.

Professional interests

Provide solutions to biological problems that demand new approaches and identify possible targets

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Contact details

New opportunities

Open to new opportunities: Yes