About
Summary
I currently worked as postdoctoral fellowship in Baruch S. Blumberg Institute (Hepatitis B Foundation) from April 2016. Currently, my research focused on understanding how the host immune system resolves the virus infection and transcriptionally inactivates covalently closed circular (ccc) DNA. And we found that two interesting ISGs (Sxx and Pxx) involved in regulation of cccDNA transcription and mediate the suppressive effect of IFN- α, at least in part, on hepadnaviral cccDNA transcription. Further mechanistic analyses demonstrated that both Sxx and Pxx are recruited to cccDNA minichromosomes and phenocopy the epigenetic modifications of cccDNA-associated histones induced by IFN-α. The manuscript was being submitted. Meanwhile, another projects focus on understanding the structural basis underlying the dual effects of CpAMs on nucleocapsid assembly / disassembly and discovery of novel therapeutic.
I earned my doctoral degree in Microbial and biochemical pharmacy from Institute of Materia Medica, Chinese Academy of Medical Science & Peking Union Medical College in July, 2015. My Ph.D. dissertation title is “CD36 is a co-receptor for hepatitis C virus E1 protein attachment and a new drug target”, which published on Scientific Report. Meanwhile, another project established wild type and lamivudine-resistant HBV replication mice model by hydrodynamic injection, which published on Acta Pharmaceutica Sinica B.
Positions
Postdoc Apr 2016 -
Hepatitis B Foundation (Baruch S Blumberg Institute)
I currently worked as postdoctoral fellowship in Baruch S. Blumberg Institute (Hepatitis B Foundation) from April 2016. Currently, my research focused on understanding how the host immune system resolves the virus infection and transcriptionally inactivates covalently closed circular (ccc) DNA. And we found that two interesting ISGs (Sxx and Pxx) involved in regulation of cccDNA transcription and mediate the suppressive effect of IFN- α, at least in part, on hepadnaviral cccDNA transcription. Further mechanistic analyses demonstrated that both Sxx and Pxx are recruited to cccDNA minichromosomes and phenocopy the epigenetic modifications of cccDNA-associated histones induced by IFN-α. The manuscript was being submitted. Meanwhile, another projects focus on understanding the structural basis underlying the dual effects of CpAMs on nucleocapsid assembly / disassembly and discovery of novel therapeutic.
Education
Peking Union Medical College 2011 - 2015
Field of study: Microbial and Biochemical Pharmacy (anti-viral)
Degree: Ph.D
With main project, we uncovered that cell membrane protein CD36 expressed on liver cells appears to directly interact with HCV E1 protein and maybe serves as a co-receptor specific for HCV attachment on and entry into host cells, blocking CD36 function with specific inhibitors as well as antibodies significantly interrupts HCV replication.
With side project, we established wild type and lamivudine-resistant HBV replication mice models by hydrodynamic injection. These models could be used to evaluate anti-HBV agents against lamivudine-resistance, affording new opportunities for establishment of other drug-resistant HBV small animal models.
Skills
Molecular and Cellular biology: Mammalian adherent and non-adherent cell culture, CRISPR-Cas9 based knock-out or knock-in, Prokaryotic and Eukaryotic expression, Cell growth inhibition assay, Immunofluorescence staining, BIAcore, ELISA, Flow cytometry, Gene clone, RNA/DNA transfection, Protein expression and purification, RNA/DNA/Protein exaction, Real-time PCR, Western blotting, Co-immunopreciptation, Chromatin immunoprecipitation. RNAseq sample preparation and data analysis.
HBV cellular biology: HBV virus preparation and titer detection, Establishment of HBV de novo infection system and HBV replicating cell lines, HBV Core DNA, hirt DNA and encapsidated pgRNA extraction, Southern blot and northern blot hybridization assay, particle assay. Induction of drug-resistant HBV small-animal model by hydrodynamic injection.
Pharmacology: HCV infection model building and drug screening techniques in vitro, Evaluation of acute toxicity in vivo, Animal intragastric administration, Intravenous injection.
Professional interests
Host-pathogen interaction; Innate immunity; Anti-viral research and related molecular mechanisms.
CV
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