About
Summary
Khalid Khan is a dedicated researcher from Pakistan with a master's degree from the University of Science and Technology of China, focusing on genes related to male infertility and cell arrest in functional genomics and cytogenetics. He utilized CRISPR-Cas9 genome editing techniques and mice as model organisms to explore the functions and pathways of these genes in genetic diseases. Currently, as an R&D team leader for cell culture, Khalid leads projects involving the differentiation of human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) into mesenchymal stem cells (MSC) using various culturing mediums and transcription factors. Additionally, he works on protein and DNA purification using silicon magnetic beads, implementing precise protocols with multiple chemicals and buffers. Khalid's strong background in genetic research and molecular biology positions him as a valuable asset in research and development roles.
Education
Abdul Wali Khan University Mardan
Field of study: Biotechnology
Degree: BS Honors
I completed my BS in Biotechnology from Abdul Wali Khan University Mardan in 2018, where my research focused on the analysis of mitochondrial DNA extracted from ancient human bones found in the local area of Swat Khazahela, KPK, Pakistan. This project provided valuable insights into the genetic heritage and historical populations of the region, allowing me to develop essential skills in molecular analysis and research methodologies.
University of Science and Technology of China
Field of study: Genetics
Degree: MS
A graduate of the University of Science and Technology of China with a master's degree, my research focused on genes associated with male infertility and cell arrest in the context of functional genomics and cytogenetics. I employed CRISPR-Cas9 genome editing techniques and mice as a model animal to study these genes, aiming to better understand their novel functions and pathways in genetic diseases like infertility.
Skills
I have four years of hands-on experience in animal care, specifically with mice, and I am skilled in genotyping and conducting various experiments on mouse tissues. My expertise includes cell culture, protein purification, mRNA expression analysis using RT and qPCR, DNA extraction, Western blotting, and immunofluorescence. I am also proficient in utilizing hematoxylin staining, scanning, and transmission electron microscopy for detailed structural analysis. Furthermore, I employ various bioinformatics tools and software for predictive analysis of gene mutations and their effects on transcriptional protein levels. My comprehensive skill set allows me to effectively contribute to complex research projects in molecular biology and genetics.
Keywords: Bioinformatics Tools, Cell Culture, DNA extraction, Gene Mutations, Genotyping , Hematoxylin Staining, hESC deifferentiation, Immunofluorescence (IF) staining, iPSC differentiation, mRNA Expression Analysis (RT and qPCR), MSC cells, PCR, Protein Purification, Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Western BlottingProfessional interests
I have a strong interest in advancing my knowledge and skills in several key areas within biology and genetics. I am particularly fascinated by the mechanisms of genetic regulation, epigenetics, and their implications in health and disease. Additionally, I am drawn to the interdisciplinary nature of aging research and its potential to uncover novel therapeutic approaches. My curiosity extends to stem cell biology, regenerative medicine, and the use of cutting-edge technologies, such as CRISPR and bioinformatics, to drive innovation in genetic research.
Keywords: Aging Research, Cell Culture, CRISPR Technology, embryonic cells, Epigenetics, Genetic Regulation, Health and Disease, iPSC differentiation, MSC cells, Regenerative medicine, Stem Cell BiologyCV
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