Khubaid Ur Rehman

Laboratory Technologist Nov 2020 -

Jaber Al Ahmad Hospital

• Prepared libraries for whole genome nanopore sequencing and sequencing data interpretation.
• Performed RNA extraction and real time PCR of Covid19 by manual and automation.
• Handled clinical samples for antibody detection of Covid19 by using serological methods.
• Registration and reporting of clinical samples by laboratory information system.
• Maintained instruments according to SOP and quality control requirements.

Senior Technician Aug 2016 - Sep 2020

Drug Regulatory Authority of Pakistan

• Analyzed the biological samples including vaccines, anti snake venom, hormones, anti cancer drugs.
• Performed cell culture, ELISA, potency testing, particle count, sterility, and general safety testing of biologicals samples.
• Handled the documents by review and record keeping of marketing authorization dossiers of biological products.
• Actively involved in the development and improvising the SOPs.

Laboratory Technician Dec 2012 - Nov 2016

Rawalpindi Institute of Cardiology

• Performed the diagnostic tests of clinical samples in pathology lab department including; microbiology, immunology, clinical pathology, hematology, transfusion medicine, and histopathology.
• Handled the instruments with their maintenance and troubleshooting.
• Maintained the documentation of record and reporting.
• Served as shift in-charge, managed the shift work and involved in training of new staff.

Laboratory Technician Jul 2009 - Dec 2012

Madina Research & Reference Lab Pvt Ltd. Rawalpindi

• Hands-on experience in phlebotomy.
• Performed diagnostic tests of clinical samples in hematology, clinical chemistry and serology departments.
• Interpreted the results and prepared the clinical reports.

COMSATS University Islamabad 2015 - 2017

Field of study: Molecular Genetics
Degree: MS

Subject studied: Molecular biology, molecular genetics, cell signaling, advanced immunology, recombinant DNA technology, biology of cancers, gene regulation and expression, research techniques.
Thesis project: Expression analysis of chloride channels in cancers.
We collected the cancerous tissue samples along with surrounding normal tissue samples and extracted the RNA and convert it into cDNA. We designed the primers by using bioinformatics tools and optimize them with our gene of interest by using conventional PCR technique followed by gel electrophoresis. After optimization, we performed the Realtime PCR to observe the expression of chloride channels in cancerous and normal control tissue samples.
Additional project: Designing of a novel filter for efficient fluoride and arsenic removal from contaminated groundwater in Pakistan.
We synthesized the iron oxide and hydroxyapatite nanoparticles and further characterized by XRD. I actively involved in designing of primers and open reading frame of plasmid by bioinformatics tools. Practically, I performed the plasmid extraction and its transformation in DH5α competent cells. Furthermore, I inoculated the transformed cells on the arsenic resistant media to observe the growth of bacteria after cloning for the efficacy of our gene of interest present in the plasmid of bacteria. Moreover, we synthesized the iron oxide and hydroxyapatite nanoparticles and further characterized by various techniques like XRD.

King Edward Medical University Lahore 2010 - 2014

Field of study: Allied Health Sciences (Medical Laboratory Technology)
Degree: BSc Hons.

Main subject studied: Microbiology, Immunology, biotechnology, hematology, transfusion medicine, chemcial pathology, clinical pathology, histology, parasitology, mycology, pharmacology.
Thesis project: Fluctuation of serum potassium in open heart surgery patients at Rawalpindi Institute of Cardiology.
This is a retrospective study in which we obtained the blood samples of post CABG patients three time per day for three consecutive days. We performed the serum potassium level in biochemistry department and observed the fluctuation of serum potassium by using various statistical assays.