About
Summary
I am an Animal's Scientist with focus in Biotechnology, mainly in genome editing in vivo and also in vitro. With a wide experience abroad, I have been worked in several different research groups in the last 10 years, applying my knowledge in molecular biology to solve problems and find solutions for challenge approaches. I am a solving-problem person by nature which means that I am willing to do what needs to be done in order to achieve a given goal.
In my CV I would like to highlight a Ms.C. degree in animal pathology, a PhD in biotechnology, a postdoctoral at University of Sao Paulo (Brazil), and short courses at NIH and EMBL, all above sponsored by Sao Paulo Government Agency (Fundacao de Amparo a Pesquisa do Estado de Sao Paulo - FAPESP). Recently I was hosted in European Molecular Biology Laboratory (EMBL - Monterotondo Unity) as a trainee, part of my first postdoctorate and nowadays I am part of staff of EMBL, in Heidelberg, Germany, where I am a EIPOD postdoc, sponsored by Marie Skłodowska-Curie Research Fellowship Programme.
A have a huge experience in cloning and vectors design for different proposals, including lentivirus and retrovirus strategies, and more recently associating this approaches with CRISPR-Cas9 system to produce knockin or knockout primary cells. I am also starting work with AAV to delivery the editing system in vivo to perform therapeutic approaches.
As part of my future plans, I would like to join a company, where I can apply my knowledges in R&D to develop new, products, process or technologies. In my target are Biotechnology and Pharmacology companies, for humans or animals issues.
Positions
Postdoc May 2016 -
Cell Biology and Biophysics, European Molecular Biology Laboratory
Project: Employing a novel organoid system to study cell polarity in breast cancer
We propose in this interdisciplinary work, to modify the current 3D acinar system established in the Jechlinger group to set up an acinar culture system that bears single, fluorescent marked, oncogenic cells in an otherwise normal mammary epithelium. Live cell imaging (SPIM technology, from Hufnagel group) will permit to trace rare events of tumorigenic outgrowth and the combination with ultrastructural 3D electron microscopy analyses (correlative light and electron microscopy, CLEM, from Schwab group) will gain detailed information on the status of epithelial junctions and cell interactions at defined points during tumor onset.
Thus, we hope to understand the interplay of oncogene activation and the cellular morphological changes caused by polarity disruption. Finally, the proposed cell culture technique is highly versatile, allowing to functionally test a large variety of genes and therefore questions in breast cancer research..
Subjects: Breast cancer; genome editing; CRISPR-Cas9; mouse; cell communication; Correlative light and electron microscopy
Postdoc Jan 2014 - Apr 2016
School of Veterinary Medicine, University of Sao Paulo
Project: The role of connexin 43 during the lung cancer development.
Tobacco in cigarettes has more than 90 known carcinogens, including NNK (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone). E10 lung cells are non-neoplastic cells, but after exposition to NNK show a higher proliferative index, higher rate of migration, greater ability to form metastases and decrease Intercellular communication mediated by Cx43 protein. This reduction in intercellular communication has been correlated with higher tumor malignancy, and worst prognosis.
In this work, our aim is to observe the role of Cx43 during the processes of tumor development and realize if restore the cell communication can hence the characteristics of malignant tumor cells.
Keywords: gap Junctions; lung cancer; gene expression by inducible system; TetOn system; carcinogenesis in vitro
PhD student Aug 2009 - Dec 2013
School of Veterinary Medicine, University of Sao Paulo
Project: Establishment and characterization of a novel transgenic mouse model with conditional expression of connexin 43 gene.
The connexins are membrane proteins that assemble the gap junctions, acting in the cell communication, and the Cx43 is the most prevalent in mouse and humans. The decrease of its expression is related with various physiological changes and its importance has been reported in the knockout mice. The complete knockout animals are not viable., so all in vivo studies about Cx43 have been performed in heterozygous animals, and then the results are correlated to try understand the role of Cx43. To improve the quality of this research field, we need a better animal model, where the gene Cx43 is completely knockout and moreover a model where we could can play with the gene expression of this gene, to understand its real role in different questions.
In this project we proposed the creation of new a transgenic model for the study of Cx43 protein. For this, we created a double transgenic model, bearing the activator gene regulated by pCx43 (pCx43-rtTA), the new gene Cx43 (pTetO-Cx43-IRES-GFP), and then this new double transgenic model was mated with the Cx43+/- animals to create our target model pCx43-rtTA; pTetO-Cx43-IRES-GFP; Cx43-/-
Keywords: Mouse; Transgenesis; Cx43; Tetracycline Inducible System
Education
University of Sao Paulo - Brazil 2009 - 2013
Field of study: Biotechnology
Degree: PhD
Establishment and characterization of a novel transgenic mouse model with conditional expression of connexin 43 gene.
University of Sao Paulo - Brazil 2006 - 2009
Field of study: Animal Pathology
Degree: Master's
Effects of Cx43 gene deletion on mouse fetal development in different genetics backgrounds: Emphasis in osteogenesis
University of Sao Paulo - Brazil 2002 - 2006
Field of study: Animal Science
Degree: Bachelor
Molecular biology applied to cancer
Skills
DNA techniques, RNA techniques, Protein techniques, Histology, Cell culture, Embryo culture, Transgenesis technology, Genome editing, Mouse experiments, Work in group, Manager team.
Professional interests
Biotechnology Companies, Pharmacology Companies, Research and Development, New Technologies, DNA Technology
CV
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