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Safia Aljedani

Postdoctoral Researcher at Fred Hutchinson Cancer Research Center

  • Joined: 2018
  • Items posted: 0
  • Profile views: 750

About

Summary

A researcher with extensive experience in structural biology, biophysics, and molecular /cell biology. Areas of research emphasis on cancer and infectious disease.

Positions

Postdoctoral Researcher Apr 2019 -

Fred Hutchinson Cancer Research Center

PhD student: Structural Biomolecular Engineering Laboratory Feb 2014 - Dec 2018

KAUST

Master student: single molecule spectroscopy and microscopy laboratory Jun 2012 - Dec 2013

KAUST

Education

KAUST 2014 - 2018

Field of study: Biological and Environmental Sciences & Engineering
Degree: Doctor of Philosophy in Bioscience/ biophysics



KAUST 2012 - 2013

Field of study: Biological and Environmental Sciences & Engineering
Degree: Master

Skills

(1) Protein expression and purification:
E. coli and mammalian systems.
Fluorescent labeling of proteins (Maleimide (Cys) and N-terminal chemistries).
Isotopic labeling of proteins for NMR (15N, 13C).
(2) Cell culture, DNA extraction, PCR, cloning.
(3) SAXS tools: ATSAS, EOM, FoXs (Fast SAXS Profile Computation), and Scatter.
(4) X-ray crystallography tools: Phenix, CCP4, Coot, MR, SAD, MAD.
(5) Insights into NMR, and CryoEM- negative staining.
(6) Biophysical assays for protein characterization:
Isothermal titration calorimetry (ITC)
Microscale thermophoresis (MST)
Dynamic light scattering (DLS).
(7) Bioinformatics tools: PyMOL, Protein modeling/ Swiss modeling, Chimera.
(8) Microscopy: confocal microscope.

Professional interests

Protein engineering.
Biophysics and biochemistry.
Structural biology (Cryo-EM, X-ray crystallography, SAXS).
Infectious diseases.
Protein-protein interaction.
Vaccine.
mRNA vaccine.

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