Shilpika Sarvepalli

Master thesis student Oct 2021 - May 2022

Lübecker Institute for Experimental Dermatology, University of Lübeck

Topic: ‘Influence of TREM1 inhibitor LR12 on Neutrophil Effector Functions’

• Planned and performed experiments independently.
•  Acquired practical skills for animal experiments (mice) and performed experiments.
• Participation in weekly lab meetings.
• Worked as a scientific assistant for the duration of the project as a part time job
• Introduced to working on Image J and MACS quantify software

Newly acquired practical skills

• Cryosection assay
• Human neutrophil isolation with polymorphprep and dextran
• Murine neutrophil isolation
• Human and murine ROS release assay
• Haematoxylin and Eosin staining
• Immunofluorescence microscopy
•  Total and specific IgG purification
• Protein estimation
   

Intern Jan 2020 - Mar 2020

Department of Infectious Diseases and Microbiology, University of Lübeck

Topic: ‘Cholesterol Homeostasis in Leishmania donovani Infected Neutrophils as Potential Targets of Host-Directed Therapies’

• ·         Acquired training and working in S2 level laboratory
• ·         Participation in weekly lab meetings
• ·         Introduced to working on Graph Pad.

Newly acquired practical skills

• Growth and culture of Leishmania donovani
• ·         Isolation of neutrophils with percoll
• ·         FACS
• ·         Limiting dilution assay

Intern Oct 2019 - Dec 2019

Bangalore

Topic: ‘Development of Aptamer Based Detection System for Detection of Shigella flexneri’

• ·         Acquired immense knowledge and practical skills in molecular biology
• ·         Participation in weekly lab meetings
• ·         Developed an aptamer independently 

Newly acquired practical skills

• ·         Asymmetric PCR
• ·         Whole Cell SELEX

Bachelor thesis student Mar 2017 - Apr 2017

PSG College of Arts & Science, PSG Institutions

·         Topic: ‘Antibiogram Study of Commercial Antibiotics Against Human Bacterial Pathogens’

• ·         Received training in microbiology.
• ·         Performed various antibiotic sensitivity tests independently

Newly acquired practical skills

• ·         Antibiotic sensitivity test
• ·         Sterilisation techniques
• ·         Bacterial plating methods

Summer trainee Mar 2016 - Mar 2016

Lucknow

• ·         Received training in basic molecular techniques, cell biology and microbiology.
• ·         Acquired all necessary skills needed for working in laboratory.

Newly acquired practical skills

• ·         Media preparation
• ·         Bacterial isolation and plating
• ·         Bacterial DNA isolation
• ·         Gram Staining and biochemical tests for bacteria
• ·         RNA isloation
• ·         Gel electrophoresis
• ·         Isolation of DNA from bacteria and blood    
• ·         SDS PAGE       
• ·         Isolation of bacterial plasmid

University of Lübeck 2018 - 2022

Field of study: Infection BIology
Degree: MSc

On the basis of pathogen-host interaction in infections, comprehensive theoretical and practical training in dealing with biological systems has been gained to be usedn other biomedical research areas. i

In addition to the scientific and technical skills, communication of science topics in the English language has been taught.

Furthermore, critically scrutinizing published scientific data, skills in documentation of own research data as well as the capability to present scientific content in an oral and written form is practiced.  Capacity for independent scientific work and acquiring team skills has been achieved.

The skills acquired by means of lectures and seminars will be trained in various practical courses and internships by solving scientific problems and designing practical, feasible solutions for current scientific questions. 

PSG College of Arts and Science, Bharathiar University 2014 - 2017

Field of study: Zoology (Life sciences)
Degree: BSc

Grasped all the necessary concepts of immunology, molecular biology, microbiology, cell biology, genetics, biochemistry and biotechnology.